CLIPS 4D

Classifier that distinguishes structurally and functionally important residue positions based on sequence and 3D data.
PDB file

Alignment file

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Instructions
Upload a PDB file. Note that the PDB-File can only contain a single chain.
Upload a multiple sequence alignment (MSA) file in FASTA format first. The first sequence in the MSA has to match the sequence in the PDB-file to allow for a mapping of residue-positions. The MSA will be filtered rigorously and must contain at least 10 sequences after filtering to allow for a sound computation of residue conservation. Please note that files larger than 15 MB are not accepted.
Here are some recommendations for creating a suitable MSA.
Here are example MSA and PDB-files.